Kamil F Tomaszewski

Follow-up the studies on K8-A1AT interaction through applying the molecules, which can reduce potential number of interaction between K8 and misfolded A1AT, and by this, enhance the secretion of mutated A1AT.

- Cells cultivation
- Cells transfection
- Protein overexpression and purification
- Co-immounoprecipitation
- In situ proximity ligation assay
- SDS-PAGE

Study of keratin 8 interaction with misfolded alpha1-antitrypsin (A1AT), its influence on A1AT secretion and processing defect in case of A1AT Deficiency disease. Investigation if this mechanism of acting is more general in the variety of loss of function diseases, like cystic fibrosis, diabetes insipidus.

- Cells cultivation
- Cells transfection and transduction
- Western blotting
- Co-immounoprecipitation
- In situ proximity ligation assay
- SDS-PAGE
- Nucleic acids purification
- PCR

Detection of leukemia-specific NPM1 mutations by using Sanger's sequencing.

Duties:

- Nucleic acids purification from blood and marrow
- PCR

Study of intracellular cell respiration by measuring energy and adenosine-5’-phosphosulfate metabolism in cultured endothelial cells through mitochondrial activity evaluation.

- Oxygraph handling
- Cells cultivating
- Cellular toxicology assay- MTT performing